RNA test could detect Alzheimer's disease
31 July 2013
A new RNA-based blood test can distinguish between people with Alzheimer's disease and healthy controls. This type of test could be used to help diagnose the disease and other degenerative disorders.
Alzheimer's disease, the most common form of dementia, can currently only be diagnosed with certainty at autopsy, so the hunt is on to find reliable, non-invasive biomarkers for diagnosis in the living. Andreas Keller and colleagues focused on microRNAs (miRNAs), small non-coding RNA molecules known to influence the way genes are expressed, and which can be found circulating in bodily fluids including blood.
The team, from Saarland University and Siemens Healthcare highlighted and tested a panel of 12 miRNAs, levels of which were found to be different amongst a small sample of Alzheimer's patients and healthy controls. In a much bigger sample, the test reliably distinguished between the two groups. The research is described in the open access journal Genome Biology.
Decent biomarkers need to be accurate, sensitive (able to correctly identify people with the disease) and specific (able to correctly pinpoint people without the disease). The new test scores over 90% on all three measures. But whilst the test shows obvious promise, it still needs to be validated for clinical use, and may eventually work best when combined with other standard diagnostic tools, such as imaging, the authors say.
As people with other brain disorders can sometimes show Alzheimer's-like symptoms, the team also looked for the miRNA signature in other patient groups. The test distinguished controls from people with various psychological disorders, such as schizophrenia and depression, with over 95% accuracy, and from patients with other neurodegenerative disorders, such as mild cognitive impairment and Parkinson's disease, with lower accuracy. It also discriminated between Alzheimer's patients and patients with other neurodegenerative disorders, with an accuracy of around 75%. But by tweaking the miRNAs used in the test, researchers think accuracy could be improved.
The work builds on previous studies highlighting the potential of miRNAs as blood-based biomarkers for many diseases, including numerous cancers, and suggests that miRNAs could yield useful biomarkers for various brain disorders. But it also sheds light on the mechanisms underpinning Alzheimer's disease. Two of the miRNAs are known to be involved in amyloid precursor protein processing, which itself is involved in the formation of plaques, a classic hallmark of Alzheimer's disease. And many of the miRNAs are believed to influence the growth and shape of neurons in the developing brain.
1. Leidinger P, et al. A blood based 12-miRNA signature of Alzheimer disease patients. Genome Biology 2013, 14:R75 doi:10.1186/gb-2013-14-7-r75 http://genomebiology.com/