MRI can non-invasively characterise brain tumours

24 March 2008

Magnetic resonance imaging (MRI) technology has the potential to non-invasively characterise tumours and determine which of them may be responsive to specific forms of treatment, based on their specific molecular properties, according to researchers at the University of California, San Diego (UCSD) School of Medicine. The study will be published online by the Proceedings of the National Academy of Science (PNAS) this week.

Normally tumour tissue and cell samples are obtained through invasive biopsy or surgery and studied under a microscope to diagnose and prescribe treatment.

“This approach reveals that, using existing imaging techniques, we can identify the molecular properties of tumours,” said Dr Michael Kuo, assistant professor of interventional radiology at UCSD School of Medicine. Kuo and colleagues analyzed more than 2,000 genes that had previously been shown to have altered expression in Glioblastoma multiforme (GBM) tumours. They then mapped the correlations between gene expression and MRI features.

The researchers also identified characteristic imaging features associated with overall survival of patients with GBM, the most common and lethal type of primary brain tumour.

The researchers discovered five distinct MRI features that were significantly linked with particular gene expression patterns. For example, one specific characteristic seen in some images is associated with proliferation of the tumour, and another with growth and formation of new blood vessels within the tumour–both of which are susceptible to treatment with specific drugs.

These physiological changes seen in the images are caused by genetic programs, or patterns of gene activation within the tumour cells. Some of these programs are tightly associated with drug targets, so when they are detected, they could indicate which patients would respond to a particular anti-cancer therapy, according to the researchers.

“For the first time, we have shown that the activity of specific molecular programs in these tumours can be determined based on MRI scans alone,” said Kuo. “We were also able to link the MRI with a group of genes that appear to be involved in tumour cell invasion — a phenotype associated with a reduced rate of patient survival.”

Laboratory work that relies on tissue samples is routinely used to diagnose and guide treatment for GBM. However, the biological activity shown may depend on the portion of the tumour from which the tissue sample is obtained. The researchers have shown that MRI could be used to identify differences in gene expression programs within the same tumour.

“Gene expression results in the production of proteins, which largely determine a tumour's characteristics and behaviour. This non-invasive MRI method could, for example, detect which part of a tumour expresses genes related to blood vessel formation and growth or tumour cell invasion,” said Kuo. “Understanding the genetic activity could prove to be a very strong predictor of survival in patients, and help explain why some patients have better outcomes than others.”

Kuo also led a study, published in Nature Biotechnology in May 2007, correlating CT images of cancerous tissue with gene expression patterns in liver tumors. “In the new study, we were able to take a different imaging technology, MRI, and apply it to a totally different tumour type,” he said, noting that the studies open up promising new avenues for non-invasive diagnoses and classification of cancer.

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