Diagnostics, nanotechnology

Concentrating viral particles in electric field could lead to rapid virus detector

9 May 2007

Engineers in the US have solved a critical bottleneck in the transport and capture of virus nanoparticles, making possible a device that could rapidly sample and detect infectious biological agents, such as viruses. They used electric fields to drive viral particles in solution to electrodes to give concentrations high enough for rapid detection.

"This advance may pave the way for an 'on-the-spot' virus detector, which would be immensely helpful, especially in military and public-health applications," said Paschalis Alexandridis, Ph.D., professor in the Department of Chemical and Biological Engineering in the University at Buffalo School of Engineering and Applied Sciences and co-author on the research.

A paper describing the results was published in the March issue of Langmuir (vol. 23, p. 3840).

The rapid detection of viruses in biological samples is of increasing interest, particularly with the recent emergence of new viruses, including SARS, West Nile virus and avian flu virus.

But because viral particles are present at such low concentrations in biological samples, such as blood, a device that can quickly and easily detect them has remained elusive.

Typical procedures involve using passive diffusion to get the viral particles to bind to an antibody, a slow process that is not feasible for many applications, such as on the battlefield, where quick results are critical.

Scientists at the University of Wisconsin at Madison led by Nicholas L. Abbott, Ph.D., a co-author on the paper and John T. and Magdalen L. Sobota Professor of Chemical and Biological Engineering, previously had demonstrated that liquid crystals can amplify signals from low concentrations of viral particles, quickly indicating whether or not a virus is present on a surface.

"The bottleneck was how to transport and capture enough suspected viral particles onto a surface in a timely fashion so that they could be detected," said Alexandridis. "During the acute phase of an infection, the virus is at a very low concentration and relying on passive diffusion to deposit the viral particles onto the detection surface can be time-consuming."

The researchers wanted to speed up the collection of viral particles — in this case, of vesicular stomatitis virus, a common animal virus — at the right place on a substrate, while also doing it in media at physiological ionic strength.

The UB researchers used their expertise in a technique called directed assembly, in which they design external electrical and fluid flow fields in order to "drive" nanoparticles to specific locations and in specific concentrations on a substrate.

"This paper shows that by using electrodes separated by just a few micrometers together with electrothermally induced fluid flow, we can accelerate the transport of viral particles from aqueous suspensions with physiological ionic strength to specific points on a surface, allowing them to reach local concentrations high enough to allow subsequent rapid detection," Alexandridis explained.

"We hypothesized that the application of these external fields would cause the nanoparticles to act in a certain way. We designed electrodes to generate the required forces for the system of interest and then put our design to the test."

In the research, the UB engineers used directed assembly to tailor dielectrophoretic forces, which act through a nonuniform electric field, overcoming an obstacle that occurs whenever nanoparticles are involved.

"When you work with microscopic objects dispersed in a liquid, gravity is very important," explained Alexandridis. "But at the nanoscale, gravity doesn't matter. So when you are trying to manipulate matter at the nanoscale, electrical fields and fluid fields may work best. By using directed assembly, we can tailor the forces acting on the nanoparticles. The ability to use several forces acting in tandem becomes important."

Electrical fields in particular, he said, are advantageous because by designing the electrodes in a certain way, engineers can control directionality and intensity of electrical forces acting on nanoparticles.

The research was conducted with colleagues from Queen's University at Kingston, Ontario, Canada and the University of Wisconsin at Madison.

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